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the control inactive reporter fopflash  (Upstate Biotechnology Inc)

 
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    Structured Review

    Upstate Biotechnology Inc the control inactive reporter fopflash
    Loss of JNK1 promoted β-catenin protein expression and β-catenin-mediated transcriptional activity <t>of</t> <t>TCF.</t> (A) β-Catenin protein expression was upregulated in JNK1−/− MEFs. Both MEFs were lysed and subjected to immunoblotting analysis detecting the expression of β-catenin and JNK1. β-Actin was used as loading control. (B) β-Catenin–TCF4 reporter activity was upregulated in JNK1−/− MEFs. JNK1+/+ and JNK1−/− MEFs were cotransfected with Renilla and a construct of TOPFLASH or <t>FOPFLASH.</t> Twenty-four hours after transfection, cells were treated with Wnt3a condition medium of 1:4 dilution for 24 h and harvested for luciferase activity assay.
    The Control Inactive Reporter Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+control+inactive+reporter+fopflash/the+control+inactive+reporter+fopflash/pmc02639245-48-6-12
    Average 90 stars, based on 1 article reviews
    the control inactive reporter fopflash - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/?-catenin signaling through GSK3? pathway"

    Article Title: c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/?-catenin signaling through GSK3? pathway

    Journal:

    doi: 10.1093/carcin/bgn239

    Loss of JNK1 promoted β-catenin protein expression and β-catenin-mediated transcriptional activity of TCF. (A) β-Catenin protein expression was upregulated in JNK1−/− MEFs. Both MEFs were lysed and subjected to immunoblotting analysis detecting the expression of β-catenin and JNK1. β-Actin was used as loading control. (B) β-Catenin–TCF4 reporter activity was upregulated in JNK1−/− MEFs. JNK1+/+ and JNK1−/− MEFs were cotransfected with Renilla and a construct of TOPFLASH or FOPFLASH. Twenty-four hours after transfection, cells were treated with Wnt3a condition medium of 1:4 dilution for 24 h and harvested for luciferase activity assay.
    Figure Legend Snippet: Loss of JNK1 promoted β-catenin protein expression and β-catenin-mediated transcriptional activity of TCF. (A) β-Catenin protein expression was upregulated in JNK1−/− MEFs. Both MEFs were lysed and subjected to immunoblotting analysis detecting the expression of β-catenin and JNK1. β-Actin was used as loading control. (B) β-Catenin–TCF4 reporter activity was upregulated in JNK1−/− MEFs. JNK1+/+ and JNK1−/− MEFs were cotransfected with Renilla and a construct of TOPFLASH or FOPFLASH. Twenty-four hours after transfection, cells were treated with Wnt3a condition medium of 1:4 dilution for 24 h and harvested for luciferase activity assay.

    Techniques Used: Expressing, Activity Assay, Western Blot, Construct, Transfection, Luciferase

    Active JNK1 downregulated β-catenin expression and inhibited its transcriptional activity. (A) Active JNK1 reduced β-catenin protein level in HEK cell line HEK293T. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1 and pcDNA3-HA-β-catenin. Forty-eight hours after transfection, cells were harvested for immunoblotting analysis to detect the alterations of HA-β-catenin, p-JNK and p-c-Jun. β-Actin served as loading control. The density of band was quantified and the expression of β-catenin was normalized to β-actin. (B) Active JNK1 inhibited β-catenin-mediated transcriptional activity of TCF4. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1, pcDNA3-HA-β-catenin, TOPFLASH or FOPFLASH and a Renilla construct. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples. Similar experiments were done in human colon cancer cell line SW480 (C and D). (E) Activated exogenous JNK1 reduced β-catenin protein level in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin and different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. The protein was obtained for immunoblotting analysis to detect the alterations of HA-β-catenin and p-JNK. β-Actin served as loading control. (F) Activated exogenous JNK1 inhibited β-catenin-mediated transcriptional activity of TCF in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin, TOPFLASH, Renilla, along with different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples.
    Figure Legend Snippet: Active JNK1 downregulated β-catenin expression and inhibited its transcriptional activity. (A) Active JNK1 reduced β-catenin protein level in HEK cell line HEK293T. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1 and pcDNA3-HA-β-catenin. Forty-eight hours after transfection, cells were harvested for immunoblotting analysis to detect the alterations of HA-β-catenin, p-JNK and p-c-Jun. β-Actin served as loading control. The density of band was quantified and the expression of β-catenin was normalized to β-actin. (B) Active JNK1 inhibited β-catenin-mediated transcriptional activity of TCF4. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1, pcDNA3-HA-β-catenin, TOPFLASH or FOPFLASH and a Renilla construct. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples. Similar experiments were done in human colon cancer cell line SW480 (C and D). (E) Activated exogenous JNK1 reduced β-catenin protein level in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin and different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. The protein was obtained for immunoblotting analysis to detect the alterations of HA-β-catenin and p-JNK. β-Actin served as loading control. (F) Activated exogenous JNK1 inhibited β-catenin-mediated transcriptional activity of TCF in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin, TOPFLASH, Renilla, along with different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples.

    Techniques Used: Expressing, Activity Assay, Transfection, Western Blot, Construct, Luciferase

    Related Articles

    Plasmid Preparation:

    Article Title: c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/?-catenin signaling through GSK3? pathway
    Article Snippet: .. TCF-4 reporter plasmid TOPFLASH and the control inactive reporter FOPFLASH were from Upstate Biotechnology (Lake Placid, NY). .. Reporter plasmid pTK-Renilla was purchased from Promega (Madison, WI).

    Control:

    Article Title: c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/?-catenin signaling through GSK3? pathway
    Article Snippet: .. TCF-4 reporter plasmid TOPFLASH and the control inactive reporter FOPFLASH were from Upstate Biotechnology (Lake Placid, NY). .. Reporter plasmid pTK-Renilla was purchased from Promega (Madison, WI).



    Similar Products

    the control inactive reporter fopflash  (Upstate Biotechnology Inc)
    90
    Upstate Biotechnology Inc the control inactive reporter fopflash
    Loss of JNK1 promoted β-catenin protein expression and β-catenin-mediated transcriptional activity <t>of</t> <t>TCF.</t> (A) β-Catenin protein expression was upregulated in JNK1−/− MEFs. Both MEFs were lysed and subjected to immunoblotting analysis detecting the expression of β-catenin and JNK1. β-Actin was used as loading control. (B) β-Catenin–TCF4 reporter activity was upregulated in JNK1−/− MEFs. JNK1+/+ and JNK1−/− MEFs were cotransfected with Renilla and a construct of TOPFLASH or <t>FOPFLASH.</t> Twenty-four hours after transfection, cells were treated with Wnt3a condition medium of 1:4 dilution for 24 h and harvested for luciferase activity assay.
    The Control Inactive Reporter Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+control+inactive+reporter+fopflash/the+control+inactive+reporter+fopflash/pmc02639245-48-6-12
    Average 90 stars, based on 1 article reviews
    the control inactive reporter fopflash - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Loss of JNK1 promoted β-catenin protein expression and β-catenin-mediated transcriptional activity of TCF. (A) β-Catenin protein expression was upregulated in JNK1−/− MEFs. Both MEFs were lysed and subjected to immunoblotting analysis detecting the expression of β-catenin and JNK1. β-Actin was used as loading control. (B) β-Catenin–TCF4 reporter activity was upregulated in JNK1−/− MEFs. JNK1+/+ and JNK1−/− MEFs were cotransfected with Renilla and a construct of TOPFLASH or FOPFLASH. Twenty-four hours after transfection, cells were treated with Wnt3a condition medium of 1:4 dilution for 24 h and harvested for luciferase activity assay.

    Journal:

    Article Title: c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/?-catenin signaling through GSK3? pathway

    doi: 10.1093/carcin/bgn239

    Figure Lengend Snippet: Loss of JNK1 promoted β-catenin protein expression and β-catenin-mediated transcriptional activity of TCF. (A) β-Catenin protein expression was upregulated in JNK1−/− MEFs. Both MEFs were lysed and subjected to immunoblotting analysis detecting the expression of β-catenin and JNK1. β-Actin was used as loading control. (B) β-Catenin–TCF4 reporter activity was upregulated in JNK1−/− MEFs. JNK1+/+ and JNK1−/− MEFs were cotransfected with Renilla and a construct of TOPFLASH or FOPFLASH. Twenty-four hours after transfection, cells were treated with Wnt3a condition medium of 1:4 dilution for 24 h and harvested for luciferase activity assay.

    Article Snippet: TCF-4 reporter plasmid TOPFLASH and the control inactive reporter FOPFLASH were from Upstate Biotechnology (Lake Placid, NY).

    Techniques: Expressing, Activity Assay, Western Blot, Construct, Transfection, Luciferase

    Active JNK1 downregulated β-catenin expression and inhibited its transcriptional activity. (A) Active JNK1 reduced β-catenin protein level in HEK cell line HEK293T. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1 and pcDNA3-HA-β-catenin. Forty-eight hours after transfection, cells were harvested for immunoblotting analysis to detect the alterations of HA-β-catenin, p-JNK and p-c-Jun. β-Actin served as loading control. The density of band was quantified and the expression of β-catenin was normalized to β-actin. (B) Active JNK1 inhibited β-catenin-mediated transcriptional activity of TCF4. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1, pcDNA3-HA-β-catenin, TOPFLASH or FOPFLASH and a Renilla construct. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples. Similar experiments were done in human colon cancer cell line SW480 (C and D). (E) Activated exogenous JNK1 reduced β-catenin protein level in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin and different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. The protein was obtained for immunoblotting analysis to detect the alterations of HA-β-catenin and p-JNK. β-Actin served as loading control. (F) Activated exogenous JNK1 inhibited β-catenin-mediated transcriptional activity of TCF in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin, TOPFLASH, Renilla, along with different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples.

    Journal:

    Article Title: c-Jun N-terminal kinase 1 interacts with and negatively regulates Wnt/?-catenin signaling through GSK3? pathway

    doi: 10.1093/carcin/bgn239

    Figure Lengend Snippet: Active JNK1 downregulated β-catenin expression and inhibited its transcriptional activity. (A) Active JNK1 reduced β-catenin protein level in HEK cell line HEK293T. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1 and pcDNA3-HA-β-catenin. Forty-eight hours after transfection, cells were harvested for immunoblotting analysis to detect the alterations of HA-β-catenin, p-JNK and p-c-Jun. β-Actin served as loading control. The density of band was quantified and the expression of β-catenin was normalized to β-actin. (B) Active JNK1 inhibited β-catenin-mediated transcriptional activity of TCF4. HEK293T cells were cotransfected with pcDNA3-Flag-MKK7-JNK1, pcDNA3-HA-β-catenin, TOPFLASH or FOPFLASH and a Renilla construct. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples. Similar experiments were done in human colon cancer cell line SW480 (C and D). (E) Activated exogenous JNK1 reduced β-catenin protein level in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin and different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. The protein was obtained for immunoblotting analysis to detect the alterations of HA-β-catenin and p-JNK. β-Actin served as loading control. (F) Activated exogenous JNK1 inhibited β-catenin-mediated transcriptional activity of TCF in a dose-dependent manner. HEK293T cells were cotransfected with pcDNA3-HA-β-catenin, TOPFLASH, Renilla, along with different amounts of pcDNA3-Flag-MKK7-JNK1, as indicated. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± SD for triplicate samples.

    Article Snippet: TCF-4 reporter plasmid TOPFLASH and the control inactive reporter FOPFLASH were from Upstate Biotechnology (Lake Placid, NY).

    Techniques: Expressing, Activity Assay, Transfection, Western Blot, Construct, Luciferase